Journal of Indian Society of Periodontology

ORIGINAL ARTICLE
Year
: 2012  |  Volume : 16  |  Issue : 4  |  Page : 549--552

Estimation of matrix metalloproteinase‑3 levels in gingival crevicular fluid in periodontal disease, health and after scaling and root planing


N Ravindra Reddy1, D Roopa1, DS Madhu Babu2, P Mohan Kumar3, C Mahendra Raju4, N Sarath Kumar1 
1 Department of Periodontics, CKS Teja Institute of Dental Sciences, Tirupati, Andhra Pradesh, India
2 Department of Periodontics, Government Dental College, RIMS, Kadapa, Andhra Pradesh, India
3 Department of Periodontics, SIBAR Institute of Dental Sciences, Guntur, Andhra Pradesh, India
4 Department of Periodontics, Sri Rama Dental College, Kanpur, Uttar Pradesh, India

Correspondence Address:
N Ravindra Reddy
Department of Periodontics, CKS Teja Institute of Dental Sciences, Tirupati, Andhra Pradesh
India

Background: Initial research has shown a positive correlation between the severity of periodontal disease and matrix metalloproteinase-3 (MMP-3) concentrations in gingival crevicular fluid (GCF). However, there are no enough reports to correlate the MMP-3 concentrations in GCF in periodontal health, disease and after treatment. Hence, the present study is to estimate the levels of MMP-3 in GCF in periodontal health, disease and to evaluate the effect of periodontal therapy on MMP-3 concentrations in GCF. Materials and Methods: Periodontal examination and collection of GCF by extracrevicular method was performed in 30 subjects selected randomly and categorized into three groups. Group I (Healthy, n=10), group II (Chronic periodontitis, n=20) and group III (After treatment group, n=20). Scaling and root planing (SRP) was performed and GCF was collected after 8 weeks of treatment. MMP-3 levels were estimated in GCF samples using enzyme linked immunosorbent assay (ELISA). Results: MMP-3 was detected in all samples. Highest mean MMP-3 concentrations in GCF were obtained for group II (7.490 ng/ml), while the lowest concentrations were seen in group I (0.344 ng/ml) and group III (2.129 ng/ml). This suggests that MMP-3 levels in GCF increases proportionally with the progression of periodontal disease and decreases after treatment. Conclusion: There is a substantial increase in the concentrations of MMP-3 as periodontal disease progresses. Since MMP-3 levels in GCF are positively correlated with gingival index, probing pocket depth and clinical attachment level, MMP-3 may be considered as a «DQ»novel biomarker«DQ» in periodontal disease progression. However, controlled, longitudinal studies are needed to confirm this possibility.


How to cite this article:
Reddy N R, Roopa D, Madhu Babu D S, Kumar P M, Raju C M, Kumar N S. Estimation of matrix metalloproteinase‑3 levels in gingival crevicular fluid in periodontal disease, health and after scaling and root planing.J Indian Soc Periodontol 2012;16:549-552


How to cite this URL:
Reddy N R, Roopa D, Madhu Babu D S, Kumar P M, Raju C M, Kumar N S. Estimation of matrix metalloproteinase‑3 levels in gingival crevicular fluid in periodontal disease, health and after scaling and root planing. J Indian Soc Periodontol [serial online] 2012 [cited 2021 Aug 1 ];16:549-552
Available from: https://www.jisponline.com/article.asp?issn=0972-124X;year=2012;volume=16;issue=4;spage=549;epage=552;aulast=Reddy;type=0