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Year : 2014  |  Volume : 18  |  Issue : 2  |  Page : 136-139  

Exploring the role of cranberry polyphenols in periodontits: A brief review

Department of Periodontics and Oral Implantology, Dr. R. Ahmed Dental College and Hospital, Kolkata, West Bengal, India

Date of Submission20-Feb-2013
Date of Acceptance07-Oct-2013
Date of Web Publication23-Apr-2014

Correspondence Address:
Malancha Mukherjee
A-2/D, Shrishti Nagar, New Asansol, Asansol - 713 305, West Bengal
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0972-124X.131301

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Cranberry juice polyphenols have gained importance over the past decade due to their promising health benefits. The bioactive component, proanthocyanidins is mainly responsible for its protective effect. A lot has been said about its role in urinary tract infection and other systemic diseases, but little is known about its oral benefits. An extensive search was carried out in the PubMed database using the terms "cranberry polyphenols" and "periodontitis" together. The institute library was also thoroughly scrutinized for all relevant information. Thus, a paper was formulated, the aim of which was to review the role of high molecular weight cranberry fraction on oral tissues and periodontal diseases.

Keywords: Cranberry, periodontitis, proanthocyanidins

How to cite this article:
Mukherjee M, Bandyopadhyay P, Kundu D. Exploring the role of cranberry polyphenols in periodontits: A brief review. J Indian Soc Periodontol 2014;18:136-9

How to cite this URL:
Mukherjee M, Bandyopadhyay P, Kundu D. Exploring the role of cranberry polyphenols in periodontits: A brief review. J Indian Soc Periodontol [serial online] 2014 [cited 2022 Aug 10];18:136-9. Available from:

   Introduction Top

Cranberry (Vaccinium macrocarpon), the native North American fruit has recently come into limelight owing to its numerous beneficial effects on health.

Widely grown in the peat bogs of the cooler north eastern part of North America, it is one of the three original fruits of the country, which grows as a vine or low trailing shrub, bears a red fruit and is acidic in taste.

In the globally functional food industry, the fruit cranberry is popularly known for its nutrient and antioxidant qualities and is extensively marketed to India and many other countries as " superfruit " in the form of fresh produce, dried fruit, juice, jams, sauces, and encapsulated powders. [1],[2]

Cranberry itself is a unique, rich source of several classes of bioactive flavonoids including flavanols, anthocyanins, and proanthocyanidins which confer it the significant therapeutic potential. The medicinal use of cranberry dates way back to the 17th century when it was a popular treatment for scurvy and gastric problems. [3]

Structure of "Proanthocyanidins"- the bioactive component of cranberries

The non- dialyzable fraction/material (NDM) produced by extensively dialyzing cranberry juice with other modifications is a product devoid of sugars and acids and contains 0.35% anthocyanins and 65.1% proanthocyanidins. [4]

Proanthocyanidins, refer to a larger class of polyphenols, called flavanols. More complex polyphenols, having the same polymeric building block, form the group of tannins.

In 1947, Prof. Jacques Masquelier, was the first to develop techniques for the extraction of proanthocyanidins from pine bark and grape seeds. [5] Proanthocyanidins are of two types: -type A found in cranberries, apricots, and peanut skin and type B, common in other berries. [6],[7]

Proanthocyanidins from cranberry juice cocktail are composed of repeating epicatechin units with at least one A-type linkage (O7 C2) as compared to the B-type linkages found in other tannin-rich foods. [8] This particular structure of cranberries is thought to influence their biological activity and in fact has been considered as a prerequisite for the antiadhesion property. [9]

In periodontal diseases, periodontopathogens colonize the teeth and subgingival area which leads to induction of protective host responses. [10]

Nevertheless incessant stimulation of the host immune system by bacteria and their products starts a chain of host-mediated destructive processes which result in the accumulation of inflammatory mediators followed by persistent inflammation frequently associated with matrix metalloproteinases (MMPs)'-mediated tissue destruction. [11],[12],[13],[14] Soluble and cell bound proteases produced by bacteria can also degrade tissue proteins and contribute to invasion of periodontal tissues. [15],[16]

Effect on caries

In oral diseases like caries and periodontitis, cranberries have shown therapeutic potential owing to their anti-adhesive and other anti-microbial properties.

An experimental work by Weiss in 1998 revealed that in vitro testing a high-molecular-weight cranberry constituent reversed the coaggregation of 49 of 84 coaggregating bacterial pairs (58%). It acted preferentially on pairs in which one or both members were gram-negative anaerobes. Thus, the anti-coaggregating cranberry constituent showed potential for altering the subgingival microbiota, resulting in adequate control of periodontal diseases. [17]

The polyphenolic fraction of the fruit reportedly repressed the formation of biofilms and acid production by cariogenic streptococci. [18],[19] Weiss and colleagues in 2004 incorporated the NDM fraction of cranberry in a mouth rinse and subjects who used it for 6 weeks reported fewer cariogenic Streptococcus mutans. [20] In yet another study, cranberry fraction demonstrated the deactivation of enzymes glucosyltranferase and fructosyltranferase. These enzymes, essential to the formation of glucan and fructan, helped the adhesion of streptococci to the tooth surface, thereby inhibiting plaque formation. [21],[22],[23]

Effect on periodontal bacteria and host mechanisms

Experimentally, Labreque in 2006 and Yamanaka in 2008 revealed that the NDM fraction of cranberry hindered the colonization of by  Porphyromonas gingivalis Scientific Name Search Fusobacterium nucleatum in the gingival crevice. Besides, it also prevented the adhesion of P. gingivalis to various proteins including type I collagen thus reducing bacterial coaggregation in periodontal diseases. [19],[24]

Cranberries were reported to restrain the proteolytic activity of the red complex specifically the gingipain activity of P. gingivalis, trypsin like activity of Tanerella forsythia and chemotrypsin like activity of T. denticola. [25] Thus, it was suggested by many workers that this fruit had the potential to limit the multiplication of pathogens by restricting their growth resources from amino acids, peptides and also inhibit the tissue destruction mediated by bacterial proteinases.

Host macrophages liberate proinflammatory cytokines (IL-1β, TNF-α) following stimulation with bacterial lipopolysaccharides of Aggregatibacter actinomycetemcomitans, F. nucleatum, P. gingivalis, T. denticola and T. forsythia. Claims like the inhibition of these cytokines were also made by Bodet et al. in 2006. [4] The fraction seemed to act by hampering the cellular signaling proteins, leading to reduction in the regulation of activator protein 1 (AP-1). This protein (AP-1) is an important transcription factor for the genes coding for proinflammatory mediators. [4],[25]

Inflammatory cells, namely macrophages and fibroblasts, produce various enzymes like MMPs and elastase which mediate tissue destruction. In vitro ELIZA study revealed that cranberry proanthocyanidins inhibited MMP responses in a dose-dependent fraction. The lipopolysaccharide-induced IL-6, IL-8, and PGE-2 responses of gingival fibroblasts were also subdued by treatment with the cranberry fraction as was Cyclooxygenase-2 expression. [26] Arrest of the catalytic activity of MMP1 and MMP9 was believed to be associated with a reduced phosphorylation of key intracellular kinases and inhibition of NF-kβ.P65 activity. [27]

Tipton et al. in 2012 suggested that cranberry components might inhibit the NF-κB and MMP-3 thus regulating aggressive periodontitis' fibroblast inflammatory responses. [28] A-type cranberry proanthocyanidins (AC-PACs) also deflect the secretion of IL-8 and chemokine ligand five (CCL5). This anti-inflammatory effect was again associated with reduced activity of the NF-kβ.p65 pathway. [29]

In 2011, Tanabe investigated the effect of AC-PACs on osteoclast formation and bone resorption activity and concluded the following. AC-PACs up to 100 µg/mL were reported to be non toxic for osteoclastic cells. Tartarase resistance acid phosphatase (TRAP) staining evidenced a dose-dependent inhibition of osteoclastogenesis. More specifically, AC-PACs at 50 µg/mL caused a 95% inhibition of RANKL-dependent osteoclast differentiation. This concentration of AC-PACs also significantly increased the secretion of IL-8 (6-fold) and inhibited the secretion of both MMP2 and MMP9. Lastly, AC-PACs affected bone degradation mediated by mature osteoclasts by significantly decreasing the release of collagen helical peptides. This study suggested that AC-PACs could interfere with osteoclastic cell maturation and physiology as well as prevent bone resorption. [30]

Other uses

The extract from the juice of cranberry has also been helpful in preventing child and female urinary tract diseases by inhibiting the adherence of  Escherichia More Details coli in the urinary tract mucosa through their high-molecular-weight tannins. [31],[32],[33]

The protective effects of cranberry juice in UTIs were once attributed to cranberry's high benzoic and quinic acid content, which the human body metabolizes into hippuric acid, a potent antibacterial. [34] While this may be a factor in cranberry's protective results, to achieve a concentration of hippuric acid in the urine that provides this bacteriostatic effect would require the average person to drink a minimum 1500 ml (over six to eight-ounce glasses) of cranberry juice per day an unlikely amount for the average person to consume. [35],[36]

Cranberry polyphenols have also been shown to deter human cancer cell line proliferation in the oral cavity, colon and prostate and reduce the risk of cardiovascular diseases. [37],[38],[39],[40]

They have arrested the development of gastric ulcers by preventing the adherence of Helicobacter pylori in the gastric mucosa and combat the adherence and infectious capacity of influenza virus. [41],[42],[43]


Different doses and formulations of cranberry, including unsweetened cranberry juice, cranberry juice cocktail, and cranberry extract tablets, have been proposed in different studies. A recent randomized controlled trial has proposed that one tablet of concentrated cranberry extract (300 to 400 mg) twice daily, or 8 oz of pure unsweetened cranberry juice three times daily for 12 months is safe and effective as an antiadhesion and antimicrobial agent for gram-negative bacteria. [44],[45]

Contraindications and adverse effects

Cranberry has a record of safety, although specific long-term safety data are lacking. No significant herb-drug interactions have been reported.

A single study found that cranberry may increase the absorption of vitamin B12 in patients who also are taking proton pump inhibitors and that it may allow the kidneys to metabolize weakly alkaline drugs (such as antidepressants and opioids) more rapidly, thus reducing their effectiveness. [46]

A small study found a significant rise in urinary oxalate levels, prompting a caution that regular use of cranberry may increase the risk of kidney stone formation in patients with a history of oxalate calculi. [47]

   Conclusion Top

Though cranberry does seem to be the "'wonder drug'" for many dental problems, it does have a few major glitches.

Drinking cranberry juice can no way be a substitute for the medical treatment or cure of UTIs, ulcers, or periodontal disease. Further study of the proanthocyanidin bioactive component of cranberry, including in vivo metabolism and bioavailability research, is necessary in order to determine the clinical applications for these compounds.

All the studies exhibiting the inhibition of bacterial adhesion or host inflammatory molecules by cranberry polyphenols have been conducted in vitro. Nothing conclusive can be said about how biological molecules may affect the same processes in the oral cavity. While Weiss and colleagues did experiment using a cranberry mouthwash which effectively inhibited caries formation, more clinical studies are undeniably required to substantiate the stated claims. The juice itself is acidic and requires sugar or other sweeteners which may contribute to demineralization and caries formation. Also, the contact time to drink a glass of cranberry juice is too short to benefit the teeth or soft tissues in any definitive manner.

Newer techniques need to be developed to incorporate the concentrated polyphenolic fraction in toothpastes, mouth rinses, and other oral hygiene products. Local drug delivery or impregnation in resorbable fibers which could be packed in the gingival sulcus may benefit the patient to some extent by preventing the destruction of host tissues due to inhibition of MMPs and other anti- inflammatory molecules.

   Acknowledgement Top

I would like to express my deepest gratitude to my co authors Dr. Debabrata Kundu and Dr. Prasanta Bandyopadhyay for their patient guidance, enthusiastic encouragement and useful critiques of this article. Their valuable and constructive suggestions during the planning and development of this article have helped me a lot. Their willingness to give time so generously has been very much appreciated.

No acknowledgement would ever be complete without thanking my parents. Their unconditional love and support has always been my strength and shaped me into the person I am today.

   References Top

1.Cranberries: How Ocean Spray made them the world's most successful superfruit. New Nutrition Business. [Last retrieved on 2011 Oct 21].  Back to cited text no. 1
2.Newsroom-'Superfruits' the future of health. HortResearch. [Last retrieved on 2009 Nov 13].  Back to cited text no. 2
3.Eck P. The American cranberry. New Brunswick, NJ: Rutgers University Press; 1990.  Back to cited text no. 3
4.Bodet C, Chandad F, Grenier D. Anti-inflammatory activity of a high-molecular-weight cranberry fraction on macrophages stimulated by lipopolysaccharides from periodontopathogens. J Dent Res. 2006;85:235-9.  Back to cited text no. 4
5.Schwitters, Bert (1995). OPC in Practice. The hidden story of proanthocyanidins, nature's most powerful and patented antioxidant (3rd ed). Alpha Omega.  Back to cited text no. 5
6.Neto, CC. Cranberry and its phytochemicals: A review of in vitro anticancer studies. J Nutr. 2007;137:186-93S.  Back to cited text no. 6
7.Lou H, Yamazaku Y, Sasaku T, Uchida M, Tanaka H, Oka S. A-type proanthocyanidins from peanut skins. Phytochemistry. 1999;51:297-308.  Back to cited text no. 7
8.Foo LY, Lu Y, Howell AB, Vorsa N. The structure of cranberry proanthocyanidins which inhibit adherence of uropathogenic P-fimbriated Escherichia coli in vitro. Phytochemistry. 2000;54:173-81.  Back to cited text no. 8
9.Howell, A. B. Bioactive compounds in cranberries and their role in prevention of urinary tract infections. Mol Nutr Food Res. 2007;51:732-7.  Back to cited text no. 9
10.Ebersole JL, Taubman MA. The protective role of host responses in periodontal diseases. Periodontol 2000. 1994;5:112-41.  Back to cited text no. 10
11.Kornman KS. Host modulation as a therapeutic strategy in the treatment of periodontal disease. Clin Infect Dis. 1999;28:520-6.  Back to cited text no. 11
12.Kinane DF, Lappin DF. Clinical, pathological and immunological aspects of periodontal disease. Acta Odontol Scand. 2001;59:154-60.  Back to cited text no. 12
13.Madianos, P. N., Bobetsis YA, Kinane DF. Generation of inflammatory stimuli: How bacteria set up inflammatory responses in the gingiva. J Clin Periodontol. 2005;32:57-71.  Back to cited text no. 13
14.Darveau RP. The Oral Microbial Consortium's Interaction with the Periodontal Innate Defense System. DNA Cell Biol. 2009;28:389-95.  Back to cited text no. 14
15.Imamura, T. The role of gingipains in the pathogenesis of periodontal disease. J Periodontol. 2003;74:111-8.  Back to cited text no. 15
16.Andrian, E., Grenier D, Rouabhia M. In vitro models of tissue penetration and destruction by Porphyromonas gingivalis. Infect Immun. 2004;72:4689-98.  Back to cited text no. 16
17.Weiss EI, Lev-Dor R, Kashamn Y, Goldhar J, Sharon N, Ofek I. Inhibiting interspecies coaggregation of plaque bacteria with a cranberry juice constituent. J Am Dent Assoc. 1998;129: 1719-23.  Back to cited text no. 17
18.Duarte S, Gregoire S, Singh AP, Vorsa N, Schaich K, Bowen WH, et al. Inhibitory effects of cranberry polyphenols on formation and acidogenicity of Streptococcus mutans biofilms. FEMS Microbiol Lett. 2006;257:50-6.  Back to cited text no. 18
19.Yamanaka-Okada A, Sato E, Kouchi T, Kimizuka R, Kato T, Okuda K. Inhibitory effect of cranberry polyphenol on cariogenic bacteria. Bull Tokyo Dent Coll 2008;49:107-12.  Back to cited text no. 19
20.Weiss EI, Kozlovsky A, Steinberg D, Lev-Dor R, Bar Ness Greenstein R, Feldman M, et al. A high molecular mass cranberry constituent reduces mutans streptococci level in saliva and inhibits in vitro adhesion to hydroxyapatite. FEMS Microbiol Lett. 2004;232:89-92.  Back to cited text no. 20
21.Banas JA, Vickerman MM. Glucan-binding proteins of the oral streptococci. Crit Rev Oral Biol Med. 2003;14:89-99.  Back to cited text no. 21
22.Koo H, Nino de Guzman P, Schobel BD, Vacca Smith AV, Bowen WH. Influence of cranberry juice on glucan-mediated processes involved in Streptococcus mutans biofilm development. Caries Res. 2006;40:20-7.  Back to cited text no. 22
23.Zero DT, Fontana M, Martínez-Mier EA, Ferreira-Zandoná A, Ando M, González-Cabezas C, et al. The biology, prevention, diagnosis and treatment of dental caries: Scientific advances in the United States. J Am Dent Assoc. 2009;140:25-34S.  Back to cited text no. 23
24.Labrecque J, Bodet C, Chandad F, Grenier D. Effects of a high molecular-weight cranberry fraction on growth, biofilm formation and adherence of Porphyromonas gingivalis. J Antimicrob Chemother. 2006;58:439-43.  Back to cited text no. 24
25.Bodet C, Piche M, Chandad F, Grenier D. Inhibition of periodontopathogen-derived proteolytic enzymes by a high-molecular-weight fraction isolated from cranberry. J Antimicrob Chemother. 2006; 57:685-90.  Back to cited text no. 25
26.Bodet C, Chandad F, Grenier D. Cranberry components inhibit interleukin-6, interleukin-8, and prostaglandin E production by lipopolysaccharide-activated gingival fibroblasts. Eur J Oral Sci. 2007;115:64-70.  Back to cited text no. 26
27.La VD, Howell AB, Grenier D. Cranberry proanthocyanidins inhibit MMP production and activity. J Dent Res. 2009;88:627-32.  Back to cited text no. 27
28.Tipton DA, Babu JP, Dabbous MK. Effects of cranberry components on human aggressive periodontitis gingival fibroblasts. J Periodontal Res. 2013;48:433-42.  Back to cited text no. 28
29.La VD, Howell AB, Grenier D. Anti-porphyromonas gingivalis and anti-inflammatory activities of A-Type cranberry proanthocyanidins. Antimicrob Agents Chemother. 2010;54:1778-84.  Back to cited text no. 29
30.Tanabe S, Santos J, La VD, Howell AB, Grenier D. A-type cranberry proanthocyanidins inhibit the RANKL-dependent differentiation and function of human osteoclasts. Molecules. 2011;16:2365-74.  Back to cited text no. 30
31.Howell AB, Reed JD, Krueger CG, Winterbottom R, Cunningham DG, Leahy M. A-type cranberry proanthocyanidins and uropathogenic bacterial anti-adhesion activity. Phytochemistry. 2005;66:2281-91.  Back to cited text no. 31
32.Ferrara P, Romaniello L, Vitelli O, Gatto A, Serva M, Cataldi L. Cranberry juice for the prevention of recurrent urinary tract infections: A randomized controlled trial in children. Scand J Urol Nephrol. 2009;9:1-5.  Back to cited text no. 32
33.McMurdo ME, Argo I, Phillips G, Daly F, Davey P. Cranberry or trimethoprim for the prevention of recurrent urinary tract infections? A randomized controlled trial in older women. J Antimicrob Chemother. 2009;63:389-95.  Back to cited text no. 33
34.Fellers, C.R., Redmon, B.C., Parrott, E.M. Effect of cranberries on urinary acidity and blood alkali reserve. J Nutr. 1933;6:455-63.  Back to cited text no. 34
35.Kahn HD, Panariello VA, Saeli J, Sampson JR, Schwartz E. Effect of cranberry juice on urine. J Am Diet Assoc. 1967;51:251-4.  Back to cited text no. 35
36.Bodel PT, Contran R, Kass EH. Cranberry juice and the antibacterial action of hippuric acid. J Lab Clin Med. 1959;54:881-8.  Back to cited text no. 36
37.Seeram NP, Adams LS, Hardy ML, Heber D. Total cranberry extract versus its phytochemical constituents: Antiproliferative and synergistic effects against human tumor cell lines. J Agric Food Chem. 2004;52:2512-7.  Back to cited text no. 37
38.Neto CC. Cranberry and blueberry: Evidence for protective effects against cancer and vascular diseases. Mol Nutr Food Res. 2007;51:652-64.  Back to cited text no. 38
39.Ferguson PJ, Kurowska E, Freeman DJ, Chambers AF, Koropatnick DJ. A flavonoid fraction from cranberry extract inhibits proliferation of human tumor cell lines. J Nutr. 2004;134:1529-35.  Back to cited text no. 39
40.McKay DL, Blumberg JB. Cranberries (Vaccinium macrocarpon) and cardiovascular disease risk factors. Nutr Rev. 2007;65:490-502.  Back to cited text no. 40
41.Burger O, Ofek I, Tabak M, Weiss EI, Sharon N, Neeman I. A high molecular mass constituent of cranberry juice inhibits Helicobacter pylori adhesion to human gastric mucus. FEMS Immunol Med Microbiol. 2000;29:295-301.  Back to cited text no. 41
42.Shmuely H, Burger O, Neeman I, Yahav J, Samra Z, Niv Y, et al. Susceptibility of Helicobacter pylori isolates to the antiadhesion activity of a high-molecular-weight constituent of cranberry. Diagn Microbiol Infect Dis. 2004;50:231-5.  Back to cited text no. 42
43.Weiss EI, Houri-Haddad Y, Greenbaum E, Hochman N, Ofek I, Zakay-Rones Z. Cranberry juice constituents affect influenza virus adhesion and infectivity. Antiviral Res. 2005;66:9-12.  Back to cited text no. 43
44.Kontiokari T, Sundqvist K, Nuutinen M, Pokka T, Koskela M, Uhari M. Randomised trial of cranberry-lingonberry juice and Lactobacillus GG drink for the prevention of urinary tract infections in women. BMJ. 2001;322:1571.  Back to cited text no. 44
45.Stothers L. A randomized trial to evaluate effectiveness and cost effectiveness of naturopathic cranberry products as prophylaxis against urinary tract infection in women. Can J Urol. 2002;9:1558-62.  Back to cited text no. 45
46.Saltzman JR, Kemp JA, Golner BB, Pedrosa MC, Dallal GE, Russell RM. Effect of hypochlorhydria due to omeprazole treatment or atrophic gastritis on protein-bound vitamin B12 absorption. J Am Coll Nutr. 1994;13:584-91.  Back to cited text no. 46
47.Terris MK, Issa MM, Tacker JR. Dietary supplementation with cranberry concentrate tablets may increase the risk of nephrolithiasis. Urology. 2001;57:26-9.  Back to cited text no. 47

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