Journal of Indian Society of Periodontology
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The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect


1 Department of Periodontics, Faculty of Dentistry, Universitas Padjadjaran, Indonesia
2 Department of Pediatrics, Faculty of Medicine, Universitas Padjadjaran, Indonesia
3 Departement of Veterinary Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia
4 Department of Oral Biology, Faculty of Dentistry, Universitas Padjadjaran, Indonesia

Correspondence Address:
Agus Susanto,
Department of Periodontics, Faculty of Dentistry Universitas, Padjadjaran, Sekeloa Selatan I, Bandung 40132
Indonesia
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jisp.jisp_232_18

Background: Collagen and chitosan are potential biomaterials for medical applications; chitosan-collagen membranes are used as a barrier membrane in guided tissue regeneration and guided bone regeneration. Aims: The purpose of this study was to analyze the effect of the chitosan-collagen membrane on wound healing in rat mandibular defect by counting the number of fibroblasts and new blood vessels. Materials and Methods: As much as 24 male Wistar rats were divided into two groups, the treatment and control group. Bone defects were made In the rat mandible with diamond bur with a diameter of 2 mm, then the defect was covered with a chitosan-collagen membrane, and the control group was covered without application of chitosan-collagen membrane. After the 3rd, 7th, 14th, and the 21st day, the defect site was analyzed histologically. The number of fibroblasts and blood vessels was counted under a light microscope, at five fields with ×1000 and ×400 microscope magnification. Statistical Analysis Used: This study was done by using analysis of variance and unpaired t-test. Results: The average number of fibroblasts and blood vessels in the treatment group was higher than the control group. There was a significant difference in the number of fibroblasts on the 3rd and 7th day (P = 0.001; P = 0.001) and the number of blood vessels on the 3rd day (P = 0.04). Conclusion: The chitosan-collagen membrane was able to increase the number of fibroblasts and new blood vessels in the wound healing process.


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    -  Susanto A
    -  Susanah S
    -  Priosoeryanto BP
    -  Satari MH
    -  Komara I
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