Journal of Indian Society of Periodontology
Journal of Indian Society of Periodontology
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ORIGINAL ARTICLE
Year : 2019  |  Volume : 23  |  Issue : 4  |  Page : 339-344

Effects of amine fluoride and probiotic mouthwash on levels of Porphyromonas gingivalis in orthodontic patients: A randomized controlled trial


1 Charitable Diagnostic Centre, Panchkula, Haryana; Department of Orthodontics and Dentofacial Orthopaedics, Sri Hasanamba Dental College and Hospital, Hassan, Karnataka, India
2 Department of Orthodontics and Dentofacial Orthopaedics, Sri Hasanamba Dental College and Hospital, Hassan, Karnataka, India
3 Department of Orthodontics and Dentofacial Orthopaedics, Malabar Dental College and Research Centre, Edappal, Kerala, India
4 Department of Public Health Dentistry, Royal Dental College, Palakkad, Kerala, India
5 Department of Periodontology, Royal Dental College, Palakkad, Kerala, India

Correspondence Address:
Dr Bobby Antony
Thiruthanathil House, Mythri-6, Thuravoor P O, Angamaly, Ernakulam - 683 572, Kerala
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jisp.jisp_551_18

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Introduction: Porphyromonas gingivalis is one among the major etiological agent in periodontal diseases and has been proved to cause gingival inflammation under orthodontic appliances. Aim: To assess the effect of amine fluoride and probiotic mouthwashes on levels of P. gingivalis during orthodontic treatment, using real time-polymerase chain reaction (RT-PCR). Materials and Methods: A randomised controlled trial was performed including 45 patients. There were three groups: Group A (control group), Group B (patients using Amflor mouthwash) and Group C (patients using probiotic mouthwash). During the treatment, all the 3 groups were advised to brush twice a day using Colgate tooth paste and Group B patients were advised to swish 15 ml fluoridated mouthwash (Amflor) and Group C advised to swish probiotic for 60 s before swallowing, once in the morning and once before sleep. After gently removing supragingival plaque, subgingival plaque samples were collected by inserting a sterile dental curette into the bottom of the gingival crevice around 31, 32, 41, and 42 at two different intervals: T1 at start and T2 after 6 months of fixed orthodontic treatment. Sub-gingival plaque samples collected were transferred to laboratory within 48 h for RT-PCR analysis in Tris-buffer solution, to maintain the integrity of bacterial DNA. Results: The levels of P. gingivalis were significantly decreased with probiotic mouth wash. Conclusions: Probiotic mouth wash can be used as an adjunctive measure along with regular brushing to improve periodontal status during fixed orthodontic treatment.


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