Journal of Indian Society of Periodontology
Journal of Indian Society of Periodontology
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Year : 2010  |  Volume : 14  |  Issue : 4  |  Page : 222-226

Viability of bacteria in dental calculus - A microbiological study

1 Department of Periodontics, Dr. D. Y. Patil Dental College, Navi Mumbai, Maharashtra, India
2 Department of Periodontics, SDM College of Dental Sciences, Dharwad , Karnataka, India
3 Department of Periodontics, Hassan Dental College, Hassan, Karnataka, India
4 Department of Microbiology, SDM Medical College, Dharwad, Karnataka, India
5 Oral Pathology SDM College of Dental Sciences, Dharwad, Karnataka, India

Correspondence Address:
Nikesh N Moolya
Department of Periodontics, Padmashree Dr. D. Y. Patil Dental College & Hospital, Nerul, Navi Mumbai - 400 706
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0972-124X.76921

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Aim: The aim of this study was (1) To investigate the viability of bacteria within supragingival and subgingival calculus, (2) To examine motility of bacteria, and (3) To identify bacterial morphotypes in calculus. Materials and Methods: Supra and subgingival calculus were harvested from 30 subjects having clinical evidence of chronic inflammatory periodontal disease and were divided into two groups . Samples from both groups were immediately transported to the Department of Microbiology for gram staining, acridine orange staining, bacterial culture and to the Department of Oral Pathology for dark field microscopy. Results: Gram staining revealed presence of bacteria within the samples. Dark field microscopic examination revealed presence of filamentous organisms, spirochetes, and motile short bacilli. Acridine orange fluorescent stain showed that the viable bacteria appeared apple green. Bacterial culture revealed presence of a variety of aerobic organisms. Conclusion: From the results, it appeared that viable bacteria were present within calculus especially within internal channels and lacunae.

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