Journal of Indian Society of Periodontology
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ORIGINAL ARTICLE
Year : 2010  |  Volume : 14  |  Issue : 2  |  Page : 109-113 Table of Contents   

Role of salivary leukocyte protease inhibitor in periodontal disease progression


1 Department of Oral Pathology, K. M. Shah Dental College and Hospital, Sumandeep Vidhyapeeth University, Vadodara, Gujarat, India
2 Department of Oral Pathology, KLE's Vishwanath Katti Institute of Dental Sciences, KLE University, Belgaum, India

Date of Submission04-Dec-2009
Date of Acceptance06-Apr-2010
Date of Web Publication2-Oct-2010

Correspondence Address:
Deepak Pateel
Department of Oral Pathology, K. M. Shah Dental College and Hospital, Sumandeep Vidhyapeeth University, Vadodara
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-124X.70830

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   Abstract 

Context: Proteases play a major role in the tissue destruction involved in periodontal disease. It is known that the balance between proteases and their inhibitors is a major determinant in maintaining tissue integrity. The association between the proteases and periodontitis is well established, but not many studies have been carried out to know the role played by a protease inhibitor like salivary leukocyte protease inhibitor (SLPI) in periodontitis. Aim: The aim of the present study was to correlate SLPI with periodontitis. Settings and Design: Case-control study. Materials and Methods: Seventy-five clinically confirmed cases of periodontitis and 20 controls were included in the study. A detailed case history and periodontal index (PI) were recorded. Two milliliters of unstimulated saliva samples was obtained and subjected to quantification of SLPI leaves using SLPI in enzyme-linked immunosorbent assay (ELISA) kit. Based on the periodontal index score of the individuals, the cases and controls were divided into groups A, B and C, and the obtained SLPI levels were compared among the groups. Statistical Analysis: Mann-Whitney U test and correlation coefficient test. Results: The results showed that in the initial stages of periodontitis there is a tendency of SLPI levels to be raised. The SLPI levels were found to be reduced in the terminal stages of periodontitis. Conclusion: It appears that SLPI accumulates in the local environment, at least in the initial stages of the periodontal disease, probably to inhibit the action of increased elastic activity.

Keywords: Chronic periodontitis, protease, saliva, SLPI


How to cite this article:
Pateel D, Seema H, Kale A. Role of salivary leukocyte protease inhibitor in periodontal disease progression. J Indian Soc Periodontol 2010;14:109-13

How to cite this URL:
Pateel D, Seema H, Kale A. Role of salivary leukocyte protease inhibitor in periodontal disease progression. J Indian Soc Periodontol [serial online] 2010 [cited 2019 Apr 22];14:109-13. Available from: http://www.jisponline.com/text.asp?2010/14/2/109/70830


   Introduction Top


Oral cavity is a unique environment. Oral mucosa is a critical protective interface between external and internal environments and serves as a barrier to the myriad microbiological species present in this warm, moist environment. The periodontium surrounding the tooth is specialized to form an attachment and seal around each tooth. This unique function imparts special challenges to the periodontium and makes it vulnerable to periodontal diseases, especially in view of continuous exposure to bacterial biofilm. [1]

Periodontitis is the most prevalent oral disease, affecting almost 90% of the population, and is also the most frequent cause of tooth loss in India. [2] Elastase plays a significant role in the connective tissue destruction associated with inflammatory processes. The free elastase activity, as well as that of other proteases, is regulated by specific inhibitors that are either produced locally or circulate in the plasma. [3] Such inhibitors include SLPI, a1 protease, a1 antitrypsin (a1-AT), a2 microglobulin, etc. [4] SLPI, a major leukocyte protease inhibitor, is an acid-stable, highly basic, nonglycosylated protein associated with a number of glandular secretions, including saliva. It is present at an average concentration of 1 to 10 μg/mL in saliva. The main functions of SLPI are as follows. [5]

  1. Protects the local tissues against determinant consequences of inflammation. It inhibits the action of proteases such as cathepsin G, elastases, trypsin, PMNs chymotrypsin and trypsin from acinar cells and chymase from mast cells.
  2. Shields the tissues against inflammatory products by down-regulating the macrophage response against bacterial lipopolysaccharide [LPS].
  3. Decreases the C5a-related chemotactic activity.
  4. SLPI has inhibitory activity against bacteria, fungi and viruses.
  5. Promotes cutaneous wound healing.


The diagnosis of active phase of periodontal disease and identification of patients at risk of active disease are challenges for clinical investigators and clinicians. Clinical indicators may reflect only past inflammation and cannot distinguish between active and inactive sites. [3] Saliva plays a protective role in maintaining oral health and consists of many locally and systemically derived factors that can be used as the basis for diagnosis of periodontal disease status. The proposed markers for diagnosing the disease status include proteins of host origin (enzymes, immunoglobulins, SLPI), phenotype markers (keratins), host cells, hormones, bacteria and bacterial products. [3]

There is fast-growing evidence that the body's own immune system is challenged by exacerbating damage caused by periodontal disease. Thus the accumulation of SLPI in the local environment may represent an intrinsic feedback mechanism to prevent harmful effects of inflammation. [7] It is generally postulated that the balance between the proteinase and anti-proteinase is a prerequisite for the maintenance of tissue integrity, and any disturbance in this equilibrium will cause active destruction of tissues. Reports have shown that the SLPI concentration is altered in various inflammatory diseases. [8] Thus the present study was carried out to compare compare between different stages of periodontitis and correlate the SLPI levels with the status or severity of periodontitis and to determine the role played by SLPI in periodontal disease progression.


   Materials and Methods Top


Source of data

The present study was carried out at our institution. The study group comprised of 75 subjects with chronic periodontitis, and control group comprised of 20 subjects with normal periodontium.

Inclusion criteria

Patients with periodontitis were chosen for the study based on the following criteria.

  1. Subjects with minimum of 20 teeth
  2. Subjects with minimum of 4 periodontal pockets
  3. Subjects with age ranging from 25 to 65 years


Exclusion criteria

Subjects less than 25 years of age.

Subjects with a history of consumption of antibiotics for the last 2 months.

Subjects with known salivary gland disease and other inflammatory lesions.

Method of collection of data

After obtaining an informed consent, a detailed case history and Russell's periodontal index (PI) were documented. Based on the PI score of the individuals, the patients and controls were divided into the following groups [Table 1].
Table 1 :Groups according to PI scores of patients and controls


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Collection of saliva samples

Patients were asked to rinse their mouth thoroughly with water. Unstimulated whole saliva was collected in a sterile bottle by asking the patient to expectorate into it gradually over a period of 5 to 10 minutes. Approximately 1-2 mL of saliva was collected.

Estimation of SLPI levels

The obtained saliva samples were stored in deepfreeze at −20C until further test was carried out. The SLPI levels were estimated by using SLPI ELISA test kit (Hycult Biotechnology). The human SLPI ELISA test kit is a solid-phase enzyme-linked immunosorbent assay based on the sandwich principle. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human SLPI. The human SLPI was captured by solid bound antibody. Unbound material present in the sample was removed by washing. Next, biotinylated second antibody tracer to human SLPI was added to the wells. In the presence of SLPI in sample, the tracer antibodies bind to the captured SLPI. The excess tracer was removed by washing. Next a streptividin-peroxidase conjugate was applied to the wells. The conjugate reacts specifically with the biotinylated tracer antibody bound onto the detected SLPI. The excess streptividin-peroxidase conjugate was removed by washing, and substrate tetramethylbenzidone (TMB) was added to the wells. Color develops proportional to the amount of human SLPI present in the sample. The enzyme reaction was stopped by the addition of citric acid, and the absorptions at 450 mm were measured with spectrophotometer.

Statistical analysis

The obtained periodontal index scores and SLPI levels in both study and control groups were tabulated, and statistical analysis was performed using Mann-Whitney U test and correlation coefficient test. Differences in mean SLPI levels between control and study groups were evaluated based on periodontal index scores of the groups with the Mann-Whitney U test and correlation coefficient test. In all the above tests, a P value of less than .05 was accepted as indicating statistical significance.


   Results Top


Maximum number of patients affected by periodontitis were in the fourth, fifth and sixth decade of their life, that is, 25.33% (19 patients) in the age group 31-40 years, 34.66% (26 patients) in age group 41-50 and 29.33% (22 patients) in age group 51-60 years. Seventy percent (14) of the controls were in the age group 41-50 years; and 30% (6), in the age group 31-40 years [Table 2].
Table 2 :Age distribution of patients and controls


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Of the 75 patients included in the study, 33.33% (40) were males and 46.66% (35) were females. In the control group, out of 20 patients, 65% (13) were males and 35% (7) were females [Table 3].
Table 3 :Gender distribution of patients and controls


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The periodontal index scores of the groups are shown in [Table 4], [Table 5], [Table 6]. A statistically significant difference with P= .086 was found in the SLPI levels between group B (terminal periodontal disease) and group C (controls) subjects. The difference in the SLPI levels between group A and group B and between group A and group C was not found to be statistically significant [Table 7].
Table 4 :Periodontal index scores and SLPI levels in study group A


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Table 5 :Periodontal index scores and SLPI levels in study group B


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Table 6 :Periodontal index scores and SLPI levels in control group


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Table 7 :SLPI levels in different groups


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   Discussion Top


In the present study, the maximum number of patients, i.e., 34.6% (26), was in the 41-50 years age group. In the control group, 70% (14) were in the 40-50 years age group [Table 2]. This is in accordance to various cross-sectional surveys, such as National Health and Nutritional Examination Surveys (NHANES 1988-1994), according to which the prevalence of chronic periodontitis and severity of attachment loss increase with age, from a low of 35.7% for 30-39 years age group to a high of 80% for 60-69 years age group. [2],[9]

In the present study, in the study group, the SLPI concentration ranged from 0 to 12 μg/mL, whereas in the control group the SLPI level ranged from 3 to 9 μg/mL [Table 4], [Table 5] and [Table 6]. Very few studies are available on the mean SLPI levels in normal subjects and in patients of periodontitis. The mean SLPI concentration reported in various studies ranged from 4 to 24 μg/mL, 1.17 μg/mL, 0.7 μg/mL and 0.2 μg/mL. [10],[11],[12],[13],[14] But the physiologic range of SLPI concentration reported in the literature is 1-10 μg/mL. [5] This variation could be due to variation in the methodology applied for the estimation of SLPI concentration in different studies.

The mean concentration of SLPI in control group was found to be 5.235 μg/mL. Among the study groups, in group A [established periodontal lesion group] the mean SLPI concentration was found to be 5.633 μg/mL; and in group B [terminal periodontal disease group], it was 4.425 μg/mL.

The mean SLPI concentration in group A was found to be increased when compared to the control group. The difference in the concentration of SLPI between group A and control group was statistically insignificant (P= .382).

Studies have established that SLPI levels are significantly increased during gingivitis and periodontitis. [10],[11] Experiments by Gillian et al. have shown that SLPI plays a critical role in the control of excessive tissue destruction and mediates wound healing. [15] Experiments have also demonstrated that the neutrophil elastase is involved in the initial destruction of periodontal ligament during early stages of periodontal disease. [16] Reports have shown that the SLPI plays an important role in controlling salivary elastase activity. [17] These findings support the findings from the present study, viz., increased SLPI levels in group A and control group when compared to group B.

The mean SLPI concentration in group B (4.425 μg/mL) [terminal periodontal disease] was significantly reduced when compared to the control group [5.325 μg/mL], with a P value of .008. Studies have reported that cathepsins and bacterial cysteine proteases are involved in the degradation of SLPI, and a proportion of SLPI would also get consumed in the resulting complex with elastase. [18],[11] So it appears that SLPI levels are decreased in the late stages of periodontitis.

The mean SLPI concentration in group A [established periodontal disease] was increased when compared to group B [terminal periodontal disease], with a P value of .1080, which was statistically not significant. Various studies have demonstrated the importance of SLPI in regulating the activity of serine proteases that are released during inflammation. Recently it has been shown that these inhibitors also play a role in tissue repair and extracellular matrix synthesis. [19] The findings, along with the reports of increased elastase activity during periodontitis, [20] indicate that our body responds to the situation by secreting more and more SLPI into the local environment during the active stages of periodontal disease.

From the results obtained in the present study, it appears that SLPI plays a role in gingivitis and periodontitis, at least in the early stages of these conditionsit appears that slpi0 plays a role in gingivitis and periodontitis, atleast in the early stages of these conditions, where active destruction of tissue is taking place. Because of its suggested antiproteolytic, antimicrobial and anti-inflammatory profiles, SLPI probably plays a protective role by maintaining a balance between proteases and antiproteases.

We have also found that SLPI levels are reduced considerably in the terminal stages of periodontitis. It will be interesting to see if analysis of SLPI levels can aid in screening of patients with terminal periodontal disease.

Finally, as host modulation as a treatment strategy in the treatment of periodontal disease is gaining importance in recent times, it appears that SLPI could possibly have a therapeutic role as it facilitates necessitates the up-regulation, inducement or enhancement of repair and wound healing in conditions such as periodontitis.


   Conclusion Top


From the results of our study, it is evident that in the initial stages of periodontitis there is a tendency of SLPI levels to be raised. It appears that SLPI accumulates in the local environment, probably to inhibit the action of increased elastic activity. It could also be due to other protective functions performed by the SLPI, like antimicrobial, anti-inflammatory, tissue repair and wound healing, during periodontal disease progression.

SLPI levels were found to be reduced in the terminal stages of periodontitis. This could be due to the degradation of SLPI by cathepsin L and bacterial cysteine protease and formation of complex with elastase.

Recommendations

Further studies are necessary to further substantiate the findings of the present study. Studies to investigate the role played by elastase, SLPI and cathepsins in both saliva and gingival cervicular fluid of periodontitis subjects are required. Large multi-center trials are needed to evaluate the role of SLPI as a host-modulating agent in the treatment of periodontitis.

 
   References Top

1.Dale BA, Fredericks LP. Antimicrobial peptides in the oral environment: expression and function in health and disease. Curr Issues Mol Biol 2005;7:119-33.  Back to cited text no. 1  [PUBMED]  [FULLTEXT]  
2.Newman TK. Caranza's Text book of Clinical Periodontology. 10 th ed. Sounders: Elsevier; 2006.  Back to cited text no. 2      
3.Kaufman E, Lamster IB. Analysis of saliva for periodontal diagnosis--a review. J Clin Periodontol 2000;27:453-65.   Back to cited text no. 3  [PUBMED]  [FULLTEXT]  
4.Figueredo CM, Gustafsson A. Activity and inhibition of elastase in GCF. J Clin Periodontol 1998;25:531-5.  Back to cited text no. 4  [PUBMED]  [FULLTEXT]  
5.Doumas S, Kolokotronis A, Stefanopoulos P. Anti-Inflammatory and Antimicrobial Roles of Secretory Leukocyte Protease Inhibitor. Infect Immun 2005;73:1271-4.   Back to cited text no. 5  [PUBMED]  [FULLTEXT]  
6.Kramps JA, Franken C, Dijkman JH. Quantity of anti-leucoprotease relative to alpha 1-proteinase inhibitor in peripheral airspaces of the human lung. Clin Sci 1988;75:351-3.   Back to cited text no. 6  [PUBMED]    
7.McNeely TB, Dealy M, Dripps DJ, Orenstein JM, Eisenberg SP, Wahl SM. In vitro Secretory leukocyte protease inhibitor: a human saliva protein exhibiting anti-human immunodeficiency virus 1 activity. J Clin Invest 1995;96:456-46.  Back to cited text no. 7  [PUBMED]  [FULLTEXT]  
8.Jorma O. Human Saliva: Clinical chemistry and Microbiology. Florida, CRC Press I1989;1:203-6.  Back to cited text no. 8      
9.Kocher T, Schwahn C, Gesch D, Bernhardt O, John U, Meisel P, et al. Risk determinants of periodontal disease-an analysis of the Study of Health in Pomerania (SHIP 0). J Clin Periodontol 2005;32:59-67.  Back to cited text no. 9  [PUBMED]  [FULLTEXT]  
10.Nakamura-Minami M, Furuichi Y, Ishikawa K, Mitsuzono-Tofuku Y, Izumi Y. Changes of alpha1-protease inhibitor and secretory leukocyte protease inhibitor levels in gingival crevicular fluid before and after non-surgical periodontal treatment. Oral Dis 2003;9:249-54.  Back to cited text no. 10  [PUBMED]  [FULLTEXT]  
11.Cox SW, Rodriguez-Gonzalez EM, Booth V, Eley BM. Secretory leukocyte protease inhibitor and its potential interactions with elastase and cathepsin B in gingival crevicular fluid and saliva from patients with chronic periodontitis. J Periodontal Res 2006;41:477-85.  Back to cited text no. 11  [PUBMED]  [FULLTEXT]  
12.Lin AL, Johnson DA, Stephan KT, Yeh CK. Salivary secretory leukocyte protease inhibitor increases in HIV infection. J Oral Pathol Med 2004;33:410-6.  Back to cited text no. 12  [PUBMED]  [FULLTEXT]  
13.Abbinante-Nissen JM, Simpson LG, Leikauf GD. Neutrophil elastase increases secretory leukocyte protease inhibitor transcript levels in airway epithelial cells. Am J Physiol 1993;265:286-92.  Back to cited text no. 13      
14.Baqui AA, Meiller TF, Falkler WA Jr. Enhanced secretory leukocyte protease inhibitor in human immunodeficiency virus type 1-infected patients. Clin Diagn Lab Immunol 1999;6:808-11.  Back to cited text no. 14  [PUBMED]  [FULLTEXT]  
15.Ashcroft GS, Lei K, Jin W, Longenecker G, Kulkarni AB, Greenwell-Wild T, Hale-Donze H, et al. Secretory leukocyte protease inhibitor mediates non-redundant functions necessary for normal wound healing. Nat Med 2000;6:1147-53.  Back to cited text no. 15  [PUBMED]  [FULLTEXT]  
16.Ujiie Y, Oida S, Gomi K, Arai T, Fukae M. Neutrophil elastase is involved in the initial destruction of human periodontal ligament. J Periodontal Res 2007;42:325-30.  Back to cited text no. 16  [PUBMED]  [FULLTEXT]  
17.Uitto VJ, Nieminen A, Coil J, Hurttia H, Larjava H. Oral fluid elastase as an indicator of periodontal health. J Clin Periodontol 1996;23:30-7.  Back to cited text no. 17  [PUBMED]    
18.Taggart CC, Lowe GJ, Greene CM, Mulgrew AT, O'Neill SJ, Levine RL, et al. Cathepsin B, L, and S cleave and inactivate secretory leucoprotease inhibitor. J Biol Chem 2001;276:33345-52.  Back to cited text no. 18  [PUBMED]  [FULLTEXT]  
19.Hiemstra PS. Novel roles of protease inhibitors in infection and inflammation. Biochem Soc Trans 2002;30:116-20.   Back to cited text no. 19  [PUBMED]  [FULLTEXT]  
20.Jin LJ, Sφder PO, Asman B, Bergstrφm K. Granulocyte elastase in gingival crevicular fluid: improved monitoring of the site-specific response to treatment in patients with destructive periodontitis. J Clin Periodontol 1995;22:240-6.  Back to cited text no. 20      



 
 
    Tables

  [Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6], [Table 7]



 

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